Today, is really a dead funny day! :D
HAHAHA!
everything was kind of quiet in the morning. and everything was dead funny after lunch! thats when everything goes bonkers & instead of feeling low becos of it, we was laughing like crazy. HAHAHA! once one of us do smthing wrong, the other will follow!
& for today, we was so blur to run a PCR that only has mastermix! LOL!!! thats becos we mix mastermix with DNA! so ended up with all mastermix. luckily, we still have DNA present. then we spoilt 1 tube of DNA! LOL lah. so now we ended up with just 1 tube of DNA. HAHAHA.
i bet today is the worse MONDAY BLACK. HAHAHA!
okay, another insider joke. :x
but it was real fun!
another long day again.
GAMBATTE!!
PPP tmr, sians, boring.
byess!
Labels: games
the more hope you have, the more disappointment you will get. :/
& this is what i had been feeling for the past 1 week.
the moment the photo come up in genesnap that captures the bands on the agarose gel, my heart just dropped to the bottom. & we had done it 4 times this week and all we get are empty photo with only DNA ladder. :/ shugs. i dont like it.
but whatever it is, we are going to do it again on monday! & i'm once again hoping it will be positive results. :D doing it as correctly as possible. (: so far only about 1 month had past for fyp and i had already learnt so many things. :D i'm happy i'm given the chance to do it. & will try my best. :P
& today, the incubator gave us a big alarm ring. LOL. seem like there is a leak in the head of the carbon dioxide tank. so, our incubator was calling not stop. irritating! & then Huay shan came and tried to solve it... then i guess she went and find the CCTA TSO! haha. and they end up doing it for more than an hour! OMGS.
then the cells look so little! i just hope nthing will happen again! :( scary leh, to have cells dying and dying. :( i hope they have the will to survive! I LOVE YOU BT549! :D LOLLLL! okay, i'm trying to be lame. HAHAHA. but yah, dont die on me again. its scary lah.
AIM FOR NOW: NUS!
FUTURE: maybe researcher, or teacher! LOL. i'm still thinking.. :/
but i like being in the lab. :)
Labels: 3rd year
A boy was watching his grandmother write a letter. At one point he asked:
‘Are you writing a story about what we’ve done? Is it a story about me?’
His grandmother stopped writing her letter and said to her grandson:
‘I am writing about you, actually, but more important than the words is the pencil I’m using. I hope you will be like this pencil when you grow up.’
Intrigued, the boy looked at the pencil. It didn’t seem very special.
‘But it’s just like any other pencil I’ve ever seen!’
‘That depends on how you look at things. It has five qualities which, if you manage to hang on them, will make you a person who is always at peace with the world.’
‘First quality: you are capable of great things, but you must never forget that there is a hand guiding your steps. We call that hand God, and He always guides us according to His will.’
‘Second quality: now and then, I have to stop writing and use a sharpner. That makes the pencil suffer a little, but afterwards, he’s much sharper. So you, too, must learn to bear certain pains and sorrows, because they will make you a better person.
‘Third quality: the pencil always allows us to use an eraser to rub out any mistakes. This means that correcting something we did is not necessarily a bad thing; it helps to keep us on the road to justice.’
‘Fourth quality: what really matters in a pencil is not its wooden exterior, but the graphite inside. So always pay attention to what is happening inside you.’
‘Finally, the pencil’s fifth quality: it always leaves a mark. in just the same way, you should know that everything you do in life will leave a mark, so try to be conscious of that in your every action’
Source: Like the Flowing River by Paulo Coelho
Labels: motivation, story
life is so full of contradictions. I felt nervous at about 6am? and i was feeling okay at ~7.30am after looking at my results. and now, while i reach home abt 30mins ago, i'm feeling down. Its as though i was about to cry, the world is going to be gone. I dont know... I want that feeling to be gone. I want to be happy again. & i know i will, cos i need to so i can WORK HARD!
this is one time i dont feel like posting up my results, even though its not my worst result, but still, its one that i could have done a lot better, but i didnt.
so, i would just say, i didnt do badly in your eyes, but i did badly in my eyes. Its so not up to my standard. but i would say this is the process of learning. :)
i'm going to work harder for the coming semester, to get into NUS! i somehow just prefer NUS more than NTU. haha. but I might only be able to get into NTU. so triple hard and more efforts needed, no more last min studying. JIAYOU FONGENG! :) WORK TOWARDS UR GOAL. :D
alright, i've enough for 1 day. enough of feelings enough of talking abt results. back to updating lab book & start on lab review report. literatures are making me super sleepy.
shall do smthing abt it then. jiayoussss. :D
& hopefully everyone is satisfied with their results. if not, work harder. :)
life is always filled with ups and downs, don't let this down bother you. reflect and work harder next time. it will be better. How much efforts is put in will be reflected on the result slip.
byes!
isnt this cute? hehes. :)
anyway, nthing much happened this week in sch for fyp other than the 2 SkBr3 cell lines that had died. :( arghs! first was due to over trypsinzed and second was due to too early subculturing resulting in no cell-cell interactions. ARGHS! :(
i hope the coming week will be better! thawing it again on Monday after all the talks. :/
and for the last day of the week, FRI, Dr. Lau showed us this movie which is related to biosafety. :) & i guess its quite a nice movie, other than the volume being too soft which ended up i dont know some parts of the show at all. but yah, overall was okay! :)
Its about a monkey that passes the virus to humans and resulting in a terrible situation. luckily at the end of the movie, everything is fine again! though part of it is not realistic. HEHE. :)
okay, i shall end my blog here! gt to update my lab record book! :D
byess!
Labels: 3rd year
say hello to mr bean! (:
okay, i'm bored! hahaha. but going to go back sch tmr so yah... just briefly updated my lab book. tmr going to do cell culture and MSP. 5 hrs, shld i bring my laptop? hmmmmms.... shall think about it.. :)
got myself a new wallet/pouch ytd, though i was suppose to get my bro's bday present! :X hehehes. i'm still bored. though i know i should enjoy it cos fyp going to start on thurs. hope everything will go smoothly!
byes!
FYP was still quite slacked cos we dont know many stuffs yet! & the cells are yet to grow finish.. & it was so CCTA!
from making solutions to cell culturing, changing medium, passaging! though things are similar, but we are so not used to it! cos now, its not u have to do, but rather, you want to do. :) so everything is still new to me! and there are many things that i dont know where is it. but so far, it has been kind of fun. & i ready have 3 cell lines growing! so its kind of scary. but so far 2 of it is still out of bound! cos its too precious for us to try out. HAHA. so its still like 1 cell line. :)
& for today, did autoclaving, some dilution of primers, watched huay shan demo changing of medium (picked up really useful TIPS that i didnt know when studying CCTA!), subculturing.
was suppose to make our own trypsin-edta, but due to us making mistake, we ended up losing the entire bottle. wasted that money! but yah, now i know to do it in the fume hood not outside! :X
then went for lunch an headed home. (:
had a nice afternoon nap cos was having a terrible headaches. :/ sighss! i hate headaches! always make me feel very tired. bleahss.
alright, time to go! wont be going to sch tmr! so ask me out if u want! haha! :) but i guess, i shld be at home reading those literature that Dr. Lau sent. (:
byebye! :D
Labels: 3rd year
this pic is great (: it talks about joy of finding something precious which was lost for a long time and thought were never getting it backed.
it let me reflect on what happened today! (a little?)
so, today was the first day of FYP. :/ its suppose to start 12th but i started mine today. and yah, didnt do much. Just briefing.
& we had to copy many many protocols. :'( then we went to print more protocols.
and i dropped my EZ-link card. LOL. and i totally didnt realised it till the student service there called me. :/ & cos i was at the canteen at that time, i couldnt really hear what they were telling me. :/ so i ended up at the library asking for my card and the people there told me had to wait till 2pm cos the other person was having lunch. LOL! so i waited, read iweekly, goggled for squash stuffs. and then went back to collect and found out actually its the student service downstairs. dots dots dots. and it was even in my horoscope, it said that i will lost something. and it did! OMGSS.
not that i'm supertitious, but it did happened! scary lehs.
so yah, i'm going to end this post here!
will be in sch tmr again & till next year. & i'm sure i'll be busy. but i still hope i will have the chance to blog and relax (:
& to SALLY, must jiayou for oiap, have fun & take care! & miss me okay! :D will miss ya tons!
byebye pple! (:
arhgs, i seriously hate RAIN! :((
now i cant go ikea becos of the rain. >:(
& i'm pissed off now. go awayy lahh!
Why is the damn rain so HEAVY!
can it be smaller. :((
aiya, dont care me, i'm just sad i cant go IKEA!haha. :P
so pardon me for this stupid post! :DDD
hahahaha~ byeeee!
& i still want to go ikea. :(
Labels: rain